Labeling of high density lipoproteins with [3H] acetic anhydride

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Labeling of high density lipoproteins with [3H] acetic anhydride.

Rat serum HDL was labeled by reaction with [3H] acetic anhydride at pH 7.2 for 30 min at room temperature by a modification of the method of Montelaro and Rueckert (1975. J. Biol. Chem. 250: 1413). Protein specific activities of 60 dpm/ng were achieved. Seven percent of the label was in lipid, of which 92 percent was recovered in phospholipid. The labeled HDL migrated as a single band as seen b...

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Simultaneous quantification of lyso-neutral glycosphingolipids and neutral glycosphingolipids by N-acetylation with [3H]acetic anhydride.

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Generation of hyperpolarized substrates by secondary labeling with [1,1-13C] acetic anhydride.

In this manuscript, the remarkable NMR signal enhancement that can be provided by dynamic nuclear polarization (DNP) was combined with the reactivity of acetic anhydride with amines to perform rapid, high SNR analyses of amino acid mixtures and to hyperpolarize new biomolecules of interest. [1,1-(13)C] acetic anhydride is an excellent substrate for DNP hyperpolarization because it can be well p...

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[High-density lipoproteins].

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1978

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)41582-2